Application
Western blots of 14-3-3 isoforms in HEK293 cell extracts using GeneTex antibodies. Lane 1 = anti-gamma 14-3-3 Lane 2 = anti-14-3-3 zeta (acetyl) antibody Lane 3 = anti-14-3-3 epsilon antibody, C-term Lane 4 = anti-14-3-3 epsilon (acetyl) antibody, N-term Lane 5 = anti-14-3-3 eta (acetyl) antibody The 14-3-3 isoforms run at 30kDa except epsilon 14-3-3 which migrates at 33kDa .| Product Name | 14-3-3 eta (acetyl) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to 14-3-3 eta (acetyl)This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and bovine orthologs. This gene contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat have been associated with early-onset schizophrenia and psychotic bipolar disorder. [provided by RefSeq, Jun 2009] |
| Immunogen | Synthetic peptide corresponding to acetylated N-terminal sequence of sheep 14-3-3 eta.Peptide sequence: Ac.GDREQLLQRARC |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| Western Blotting: Max Dilution 1/3000: |
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| Species Reactivity | HumanMouseChickenRatRabbitSheepBovine |
| Predicted Reactivity | Mammal |
| Concentration | 1mg/ml |
| Purification | Serum |
| Gene Synonyms | YWHA1 YWHAH tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein eta polypeptide |
|---|---|
| Target | 14-3-3 eta (acetyl) |
| Tissue Specificity | This antibody recognizes 14-3-3 eta in all mammals. It may not react with recombinant proteins that are not N-acetylated. |
Application
Western blots of 14-3-3 isoforms in HEK293 cell extracts using GeneTex antibodies. Lane 1 = anti-gamma 14-3-3 Lane 2 = anti-14-3-3 zeta (acetyl) antibody Lane 3 = anti-14-3-3 epsilon antibody, C-term Lane 4 = anti-14-3-3 epsilon (acetyl) antibody, N-term Lane 5 = anti-14-3-3 eta (acetyl) antibody The 14-3-3 isoforms run at 30kDa except epsilon 14-3-3 which migrates at 33kDa .| Application Notes | Western Blotting: Max Dilution 1/3000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Storage Buffer | 0.09%Sodium Azide (NaN3) |
