Application
Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE diluted at 1:1000Application
14-3-3 eta antibody detects 14-3-3 eta protein at cytoplasm on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. 14-3-3 eta antibody diluted at 1:500.Application
Immunofluorescence analysis of methanol-fixed HeLa, using 14-3-3 eta antibody at 1:500 dilution.| Product Name | 14-3-3 eta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to 14-3-3 eta (tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta polypeptide)This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and bovine orthologs. This gene contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat has been associated with early-onset schizophrenia. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human 14-3-3 eta. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Rabbit, Chicken, Rhesus Monkey, BovineMouse (100%), Rat (100%), Rabbit (100%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | YWHA1 antibody YWHAH antibody 14-3-3 protein eta antibody 14-3-3 eta antibody "tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein eta polypeptide antibody" |
|---|---|
| Molecular Weight(MW) | 28 kDa (note) |
Application
Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE diluted at 1:1000
Application
14-3-3 eta antibody detects 14-3-3 eta protein at cytoplasm on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. 14-3-3 eta antibody diluted at 1:500.
Application
Immunofluorescence analysis of methanol-fixed HeLa, using 14-3-3 eta antibody at 1:500 dilution.| Positive Control | HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
