Application
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:10000Application
Sample (30 ug of whole cell lysate) A: Hep G2 12% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of methanol-fixed HeLa, using 14-3-3 gamma antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using 14-3-3 gamma antibody at 1:500 dilution.| Product Name | 14-3-3 gamma antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to 14-3-3 gamma (tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide)This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human 14-3-3 gamma. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Chicken, Rhesus Monkey, BovineRat (100%), Xenopus laevis (98%), Chicken (99%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 14-3-3GAMMA antibody YWHAG antibody KCIP-1 antibody 14-3-3 gamma antibody 14-3-3 protein gamma antibody protein kinase C inhibitor protein 1 antibody "tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma polypeptide antibody" |
|---|---|
| Molecular Weight(MW) | 28 kDa (note) |
| Cellular Localization | Cytoplasm |
Application
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:10000
Application
Sample (30 ug of whole cell lysate) A: Hep G2 12% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of methanol-fixed HeLa, using 14-3-3 gamma antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using 14-3-3 gamma antibody at 1:500 dilution.| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
