Application
IHC-P analysis of human breast carcinoma using 4E-BP1 (phospho Thr36) antibody.Application
WB analysis of extracts from untreated and EGF-treated MDA-MB-435 cells using 4E-BP1 (phospho Thr36) antibody.| Product Name | 4E-BP1 (phospho Thr36) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to 4E-BP1 (phospho Thr36)This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008] |
| Immunogen | A phospho specific peptide corresponding to residues surrounding T36 of human EIF4EBP1 |
| Modification | p-Thr36 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB: 1:500 - 1:2000. IHC: 1:50 - 1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BP-1 4E-BP1 4EBP1 eukaryotic translation initiation factor 4E binding protein 1 EIF4EBP1 PHAS-I |
|---|---|
| Target | 4E-BP1 (phospho Thr36) |
| Molecular Weight(MW) | 18 kDa (note) |
Application
IHC-P analysis of human breast carcinoma using 4E-BP1 (phospho Thr36) antibody.
Application
WB analysis of extracts from untreated and EGF-treated MDA-MB-435 cells using 4E-BP1 (phospho Thr36) antibody.| Application Notes | WB: 1:500 - 1:2000. IHC: 1:50 - 1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
