Application
Western blot analysis of extracts from Hela cells using 4E-BP1(Phospho-Thr45) Antibody and the same antibody preincubated with blocking peptide.Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4E-BP1 (Phospho-Thr45) Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from Hela cells using 4E-BP1 (Phospho-Thr45) Antibody and the same antibody preincubated with blocking peptide.| Product Name | 4E-BP1 (phospho Thr45) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to 4E-BP1 (Phospho Thr45)4E-BP1 encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. |
| Immunogen | Peptide sequence around phosphorylation site of threonine 45 (S-T-T(p)-P-G) derived from Human 4E-BP1. |
| Modification | p-Thr45 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 4E-BP1 4EBP1 BP-1 MGC4316 PHAS-I EIF4EBP1 eukaryotic translation initiation factor 4E-binding protein 1 eIF4E-binding protein 1 phosphorylated heat- and acid-stable protein regulated by insulin 1 eukaryotic translation initiation factor 4E binding |
|---|---|
| Target | 4E-BP1 (Phospho Thr45) |
| Molecular Weight(MW) | 18 (note) |
Application
Western blot analysis of extracts from Hela cells using 4E-BP1(Phospho-Thr45) Antibody and the same antibody preincubated with blocking peptide.
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4E-BP1 (Phospho-Thr45) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from Hela cells using 4E-BP1 (Phospho-Thr45) Antibody and the same antibody preincubated with blocking peptide.| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
