Application
Immunocytochemistry of 4E-BP1 in 3T3 cells with 4E-BP1 antibody at 2 ug/mL.Application
Western blot analysis of 4E-BP1 in 3T3 cell lysate with 4E-BP1 antibody at (A) 2.5, (B) 5 and (C) 10 ug/mL.| Product Name | 4E-BP1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to 4E-BP1This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008] |
| Immunogen | 4E-BP1 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human 4E-BP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB: 2.5 - 10 ug/ml. ICC: 2 ug/ml: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PHAS-I EIF4EBP1 4EBP1 BP-1 eukaryotic translation initiation factor 4E binding protein 1 |
|---|---|
| Target | 4E-BP1 |
Application
Immunocytochemistry of 4E-BP1 in 3T3 cells with 4E-BP1 antibody at 2 ug/mL.
Application
Western blot analysis of 4E-BP1 in 3T3 cell lysate with 4E-BP1 antibody at (A) 2.5, (B) 5 and (C) 10 ug/mL.| Application Notes | WB: 2.5 - 10 ug/ml. ICC: 2 ug/ml: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Be stored at 4oC for three months and -20oC, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PBS containing 0.02% sodium azide. |
