Application
Western blot detection of 53BP1 (220 kDa) from untreated U2OS cell lysate usingApplication
53BP1 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 594 (red).Application
IHC analysis of 53BP1 in human renal cancer using DAB with hematoxylin counterstain.| Product Name | 53BP1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody cocktail to 53BP1 (p53 Binding Protein 1)P53-binding protein 1 (53BP1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, it is phosphorylated and relocalizes to the presumptive sites of damage, specifically, double-strand breaks. This also suggests a role in DNA repair, maintaining genomic stability. |
| Immunogen | A synthetic peptide, which represented a portion of human tumor protein p53-binding protein 1 encoded in exon 11, 12 and 19 (LocusLink ID 7158). This antibody is a cocktail hybrid. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PWB |
| Immunocytochemistry - 1:100-1:1,000 : |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 53BP1 MGC138366 TP53BP1 p202 7158 605230 Q12888 |
|---|---|
| Target | 53BP1 |
| Tissue Specificity | is specific for human 53BP1 protein. |
Application
Western blot detection of 53BP1 (220 kDa) from untreated U2OS cell lysate using
Application
53BP1 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 594 (red).
Application
IHC analysis of 53BP1 in human renal cancer using DAB with hematoxylin counterstain.| Positive Control | U205 cell lysates |
|---|---|
| Application Notes | Immunocytochemistry - 1:100-1:1,000 : |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | Tris-citrate/phosphate (pH 7-8) buffer with 0.1% Sodium Azide. |
