Application
Western blot analysis of AAK1 expression in 3T3 cell lysate (Lane 1) and rat Kidney tissue lysate (Lane 2).| Product Name | AAK1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to AAK1AAK1 is a serine/threonine kinase that is thought to coordinate the recruitment of AP-2 to receptors containing tyrosine-based internalization motifs by phosphorylating the μ2 subunit. There is strong evidence that this protein is the endogenous mu 2 kinase and plays a regulatory role in clathrin-mediated endocytosis, including the regulation of fatty acid synthesis and cholesterol synthesis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~95 kDa AAK1 in samples from human, mouse and rat origins. Reactivity to other species has not been tested.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AKK 1 AP2 associated kinase 1 AAK-1 Adaptor associated kinase 1 AP2 associated kinase1 ZNF630 AAK1 AKK1 Adaptor associated kinase1 AAK 1 |
|---|---|
| Target | AAK1 |
| Tissue Specificity | This antibody is specific for AAK1. |
Application
Western blot analysis of AAK1 expression in 3T3 cell lysate (Lane 1) and rat Kidney tissue lysate (Lane 2).| Application Notes | Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~95 kDa AAK1 in samples from human, mouse and rat origins. Reactivity to other species has not been tested.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C.The antibody solution should be gently mixed before use. |
| Storage Buffer | PBS (pH 7.2) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal |
