Application
Western blot analysis of extracts of various cell lines, using ABAT antibody.Application
Immunofluorescence analysis of A549 cell using ABAT antibody. Blue: DAPI for nuclear staining.Application
Immunohistochemistry of paraffin-embedded human gastric cancer using ABAT antibody at dilution of 1:100 (x40 lens).Application
Immunohistochemistry of paraffin-embedded human liver injury using ABAT antibody at dilution of 1:100 (x40 lens).Application
Immunohistochemistry of paraffin-embedded rat kidney using ABAT antibody at dilution of 1:100 (x40 lens).| Product Name | ABAT antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ABAT4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant protein of human ABAT |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PWB |
| WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 4-aminobutyrate aminotransferase GABAT NPD009 GABA-AT |
|---|---|
| Target | ABAT |
| Molecular Weight(MW) | 56 kDa (note) |
Application
Western blot analysis of extracts of various cell lines, using ABAT antibody.
Application
Immunofluorescence analysis of A549 cell using ABAT antibody. Blue: DAPI for nuclear staining.
Application
Immunohistochemistry of paraffin-embedded human gastric cancer using ABAT antibody at dilution of 1:100 (x40 lens).
Application
Immunohistochemistry of paraffin-embedded human liver injury using ABAT antibody at dilution of 1:100 (x40 lens).
Application
Immunohistochemistry of paraffin-embedded rat kidney using ABAT antibody at dilution of 1:100 (x40 lens).| Application Notes | WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
