Application
Western blot analysis of ABCB5 Antibody (N-term) in A375, K562, A2058 and HL-60 cell line lysates (35ug/lane). ABCB5 (arrow) was detected using the purified Pab.Application
Flow Cytometry using ABCB5 Antibody (N-Term) on WM852 cell line. Live cell staining utilized PE-conjugated goat anti-rabbit (Jackson ImmunoResearch) as a secondary antibody.?Analysis was done on an FC500 flow cytometer. Data courtesy of Dr. Steve Reuland, University of Colorado, DenverApplication
Flow Cytometry using ABCB5 Antibody (N-Term) on SkMel-30 cell line. Live cell staining utilized PE-conjugated goat anti-rabbit (Jackson ImmunoResearch) as a secondary antibody.?Analysis was done on an FC500 flow cytometer. Data courtesy of Dr. Steve Reuland, University of Colorado, Denver| Product Name | ABCB5 antibody, N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to ABCB5 (N-term)ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules (Chen et al., 25 [PubMed 1576339]).[supplied by OMIM] |
| Immunogen | KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human ABCB5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Gene Synonyms | ABCB5 P-gp Pglycoprotein ABCB5 ABCB5 ATPbinding cassette subfamily B member 5 ATPbinding cassette subfamily B member5 P-glycoprotein ABCB5 ATP-binding cassette sub-family B member 5 ABCB5 Pgp ABCB5 P gp P glycoprotein ABCB5 |
|---|---|
| Target | ABCB5 |
| Molecular Weight(MW) | 89.831 kDa (note) |
Application
Western blot analysis of ABCB5 Antibody (N-term) in A375, K562, A2058 and HL-60 cell line lysates (35ug/lane). ABCB5 (arrow) was detected using the purified Pab.
Application
Flow Cytometry using ABCB5 Antibody (N-Term) on WM852 cell line. Live cell staining utilized PE-conjugated goat anti-rabbit (Jackson ImmunoResearch) as a secondary antibody.?Analysis was done on an FC500 flow cytometer. Data courtesy of Dr. Steve Reuland, University of Colorado, Denver
Application
Flow Cytometry using ABCB5 Antibody (N-Term) on SkMel-30 cell line. Live cell staining utilized PE-conjugated goat anti-rabbit (Jackson ImmunoResearch) as a secondary antibody.?Analysis was done on an FC500 flow cytometer. Data courtesy of Dr. Steve Reuland, University of Colorado, Denver| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
