Application
Sample (30 ug of whole cell lysate) A: 293T 5% SDS PAGE diluted at 1:3000Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using ABCF1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using ABCF1 antibody at 1:500 dilution.| Product Name | ABCF1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ABCF1 (ATP-binding cassette, sub-family F (GCN20), member 1)The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the GCN20 subfamily. Unlike other members of the superfamily, this protein lacks the transmembrane domains which are characteristic of most ABC transporters. This protein may be regulated by tumor necrosis factor-alpha and play a role in enhancement of protein synthesis and the inflammation process. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human ABCF1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Rhesus Monkey, Chimpanzee, BovineRhesus Monkey (97%), Chimpanzee (98%), Bovine (83%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ABC27 antibody ABC50 antibody ABCF1 antibody ATP-binding cassette 50 (TNF-alpha stimulated) antibody TNF-alpha-stimulated ABC protein antibody TNFalpha-inducible ATP-binding protein antibody ATP-binding cassette sub-family F member 1 antibody "ATP-b |
|---|---|
| Molecular Weight(MW) | 96 kDa (note) (3) |
Application
Sample (30 ug of whole cell lysate) A: 293T 5% SDS PAGE diluted at 1:3000
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using ABCF1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using ABCF1 antibody at 1:500 dilution.| Positive Control | 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
