Rabbit Polyclonal antibody to ABCG2Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123.
Immunogen
Peptide sequence around aa.160~164( R-I-N-R-V) derived from Human ABCG2(CD338).
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
WB
Suggested starting dilutions are as follows. WB: 1:500. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:
Species Reactivity
HumanMouseRat
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Purification
Affinity purified
Target Information
Gene Synonyms
ABC15 ABCP BCRP BCRP1 BMDP CD338 CDw338 EST157481 MGC102821 MRX MXR MXR1 ABCG2 ATP-binding cassette sub-family G member 2 mitoxantrone resistance-associated protein breast cancer resistance protein ATP-binding cassette transporter G2 place
Target
ABCG2
Molecular Weight(MW)
65-80 (note)
Application
Application
Western blot analysis of extract from HL-60 cells using ABCG2(CD338) Antibody
Application
Western blot analysis of extract from HL-60 cells using ABCG2 (CD338) Antibody
Application Notes
Suggested starting dilutions are as follows. WB: 1:500. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.