Application
Immunohistochemistry of ABIN3 in mouse spleen tissue with ABIN3 antibody at 5 ug/ml.Application
Western blot analysis of ABIN3 in human spleen tissue lysate with ABIN3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.Application
Western blot analysis of ABIN3 in human spleen tissue lysate with ABIN3 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.| Product Name | ABIN3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to ABIN3The nuclear factor NF-kB plays key roles in development and immunity. ABIN3 (A20-binding inhibitor of NF-kB activation 3), also known as TNFAIP3-interacting protein 3 (TNIP3), is a novel negative feedback regulator of LPS-induced NF-kB activation. ABIN3 is a 39 kDa protein that negatively regulates NF-kB activation in response to TNF and LPS. ABIN3 is highly expressed in brain, thymus, lymph node, lung and fetal liver, with low expression in kidney, bone marrow. Through its interaction with A20, ABIN3 interferes with TRAF2-mediated transactivation signals and NF-kB inhibition is mediated by the ABIN-homology domain 2. ABIN3 has been found to be induced by Listeria infection and can be slightly downregulated by dexamethasone. Enhanced expression of ABIN3 in monocytes is associated with sepsis. Thus, ABIN3 is an IL-10-induced gene product capable of attenuating NF-kB in human macrophages yet is inoperative in mice and represents a basis for species-specific differences in IL-10 actions. At least four isoforms of ABIN3 are known to exist. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | A20binding inhibitor of NFkappaB activation3 A20-binding inhibitor of NF-kappaB activation 3 TNIP3 ABIN3 TNFAIP3 interacting protein 3 Listeria-induced gene protein TNFAIP3 interacting protein3 LIND A20binding inhibitor of NFkappaB activation 3 Li |
|---|---|
| Target | ABIN3 |
| Tissue Specificity | This antibody is specific for ABIN3 |
Application
Immunohistochemistry of ABIN3 in mouse spleen tissue with ABIN3 antibody at 5 ug/ml.
Application
Western blot analysis of ABIN3 in human spleen tissue lysate with ABIN3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Application
Western blot analysis of ABIN3 in human spleen tissue lysate with ABIN3 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.| Positive Control | Blocking Peptide: |
|---|---|
| Application Notes | IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.02% sodium azide |
