Application
Immunofluorescence staining of methanol-fixed Hela cells using ABL1/2(phospho-Tyr393/439) AntibodyApplication
Western blot analysis of extracts from HL60 cells using ABL1/2(phospho-Tyr393/439) Antibody and the same antibody preincubated with blocking peptide.Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ABL1/2(Phospho-Tyr393/439) Antibody (left) or the same antibody incubated with blocking peptide (right)| Product Name | ABL1/2 (Phospho Tyr393/439) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ABL1/2 (Phospho Tyr393/439)Regulates cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. Localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics. Regulates DNA repair potentially by activating the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks. |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 393/439 (D-T-Y(p)-T-A) derived from Human ABL1/2. |
| Modification | p-Tyr393/439 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ABL JTK7 bcr/abl c-ABL p150 v-abl ABL1 proto-oncogene tyrosine-protein kinase ABL1 Abelson tyrosine-protein kinase 1 proto-oncogene c-Abl v-abl Abelson murine leukemia viral oncogene homolog 1 c-abl oncogene 1 receptor tyrosine kinase tyrosine |
|---|---|
| Target | ABL1/2 (Phospho Tyr393/439) |
| Molecular Weight(MW) | 210 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using ABL1/2(phospho-Tyr393/439) Antibody
Application
Western blot analysis of extracts from HL60 cells using ABL1/2(phospho-Tyr393/439) Antibody and the same antibody preincubated with blocking peptide.
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ABL1/2(Phospho-Tyr393/439) Antibody (left) or the same antibody incubated with blocking peptide (right)| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
