Application
Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using ACADM antibody at 1:100 dilution.Application
Sample(30 μg of whole cell lysate) A:HeLa S3 10% SDS PAGE diluted at 1:1000Application
ACADM antibody detects ACADM protein at mitochondria by immunofluorescent analysis. Sample: SKNSH cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: ACADM protein stained by ACADM antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | ACADM antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to ACADM (acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain)This gene encodes the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Defects in this gene cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ACADM. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | ChimpanzeeChimpanzee (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ACAD1 antibody FLJ18227 antibody FLJ93013 antibody FLJ99884 antibody MCAD antibody MCADH antibody ACADM antibody "acyl-Coenzyme A dehydrogenase C-4 to C-12 straight chain antibody" "medium-chain specific acyl-CoA dehydrogenase mitochondrial antib |
|---|---|
| Target | ACADM |
| Molecular Weight(MW) | 47 kDa (note) (5) |
| Cellular Localization | Mitochondrion matrix |
Application
Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using ACADM antibody at 1:100 dilution.
Application
Sample(30 μg of whole cell lysate) A:HeLa S3 10% SDS PAGE diluted at 1:1000
Application
ACADM antibody detects ACADM protein at mitochondria by immunofluorescent analysis. Sample: SKNSH cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: ACADM protein stained by ACADM antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | HeLaS3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
