Application
Immunohistochemical analysis of paraffin-embedded H358 xenograft, using ACADVL antibody at 1:250 dilution.Application
Sample (50 µg of whole cell lysate) A: mouse liver 7.5% SDS PAGE diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: HeLa 7.5% SDS PAGE VLCAD antibody diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
ACADVL antibody [N1C1] detects ACADVL protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded human A549 xenograft. ACADVL antibody [N1C1] diluted at 1:500.Application
ACADVL antibody [N1C1] detects ACADVL protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ACADVL protein stained by ACADVL antibody [N1C1] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.| Product Name | ACADVL antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ACADVL (acyl-Coenzyme A dehydrogenase, very long chain)The protein encoded by this gene is targeted to the inner mitochondrial membrane where it catalyzes the first step of the mitochondrial fatty acid beta-oxidation pathway. This acyl-Coenzyme A dehydrogenase is specific to long-chain and very-long-chain fatty acids. A deficiency in this gene product reduces myocardial fatty acid beta-oxidation and is associated with cardiomyopathy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ACADVL. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, BovineRat (88%), Zebrafish (81%), Bovine (91%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ACAD6 antibody LCACD antibody VLCAD antibody ACADVL antibody "very long-chain specific acyl-CoA dehydrogenase mitochondrial antibody" "acyl-Coenzyme A dehydrogenase very long chain antibody" "acyl-CoA dehydrogenase very long chain antibody" |
|---|---|
| Molecular Weight(MW) | 70 kDa (note) (3) |
| Cellular Localization | Mitochondrion inner membrane |
Application
Immunohistochemical analysis of paraffin-embedded H358 xenograft, using ACADVL antibody at 1:250 dilution.
Application
Sample (50 µg of whole cell lysate) A: mouse liver 7.5% SDS PAGE diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: HeLa 7.5% SDS PAGE VLCAD antibody diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
ACADVL antibody [N1C1] detects ACADVL protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded human A549 xenograft. ACADVL antibody [N1C1] diluted at 1:500.
Application
ACADVL antibody [N1C1] detects ACADVL protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ACADVL protein stained by ACADVL antibody [N1C1] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.| Positive Control | H1299 , HeLa , HepG2 , mouse liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
