Application
ACAT1 antibody [N1N3] detects ACAT1 protein at cytoplasm on human normal kidney by immunohistochemical analysis. Sample: Paraffin-embedded human normal kidney. ACAT1 antibody [N1N3] diluted at 1:500.Application
ACAT1 antibody [N1N3] detects ACAT1 protein at mitochondria by immunofluorescent analysis. Sample: A431 cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: ACAT1 protein stained by ACAT1 antibody [N1N3] diluted at 1:500. Blue: Hoechst 33342 staining.Application
Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000Application
Sample(30 μg of whole cell lysate) A:293T B:A431 C:MOLT4 10% SDS PAGE diluted at 1:500Application
ACAT1 antibody [N1N3] detects ACAT1 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ACAT1 protein stained by ACAT1 antibody [N1N3] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.| Product Name | ACAT1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ACAT1 (acetyl-Coenzyme A acetyltransferase 1)This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human ACAT1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Rhesus Monkey, BovineRat (83%), Rhesus Monkey (98%), Bovine (95%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ACAT antibody MAT antibody T2 antibody THIL antibody ACAT1 antibody mitochondrial acetoacetyl-CoA thiolase antibody acetoacetyl-CoA thiolase antibody acetyl-Coenzyme A acetyltransferase 1 antibody "acetyl-CoA acetyltransferase mitochondrial antibo |
|---|---|
| Molecular Weight(MW) | 45 kDa (note) |
| Cellular Localization | Mitochondrion |
Application
ACAT1 antibody [N1N3] detects ACAT1 protein at cytoplasm on human normal kidney by immunohistochemical analysis. Sample: Paraffin-embedded human normal kidney. ACAT1 antibody [N1N3] diluted at 1:500.
Application
ACAT1 antibody [N1N3] detects ACAT1 protein at mitochondria by immunofluorescent analysis. Sample: A431 cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: ACAT1 protein stained by ACAT1 antibody [N1N3] diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000
Application
Sample(30 μg of whole cell lysate) A:293T B:A431 C:MOLT4 10% SDS PAGE diluted at 1:500
Application
ACAT1 antibody [N1N3] detects ACAT1 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ACAT1 protein stained by ACAT1 antibody [N1N3] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.| Positive Control | 293T , A431 , Molt-4 , mouse brain , *F31 , *F90 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
