Application
Immunofluorescence of ACE2 in Human Kidney cells with ACE2 antibody at 10 ug/mL.Application
Western blot analysis of ACE2 in human kidney lysate with ACE2 antibody at 0.5 (lane A), 1 (lane B), and 2 (lane C) μg/mL, respectively.Application
Immunohistochemical staining of human kidney tissue using ACE2 antibody at 2 μg/mL.| Product Name | ACE2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to ACE2Angiotensin converting enzyme 2 (ACE2) plays a central role in vascular, renal, and myocardial physiology. In contrast to its homolog ACE, ACE2 expression is restricted to heart, kidney, and testis. Recently ACE2 has also been shown to be a functional receptor of the SARS coronavirus. The normal function of ACE2 is to convert the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1-7, unlike ACE, which converts AngI to AngII. While the role of these vasoactive peptides is not well understood, lack of ACE2 expression in ace2-/ace2- mice leads to severely reduced cardiac contractility, indicating its importance in regulating heart function. ACE1 inhibitors such as catopril and lisinopril do not block ACE2 activity. ACE2 is restricted to the testis, heart, kidney and colon. The full length sequence for ACE2 encodes an 805 amino acid protein with predicted mass of 92.46 kDa and a pI of 5.22. No cross reactivity to ACE1. |
| Immunogen | Synthetic peptide corresponding to C-terminal amino acids 788-805 of human ACE2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-FIHC-PWB |
| For IHC-P: Use at a concentration of 2ug/ml. WB: Use at a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 97 kDa). Peptide available as GTX15352. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Q9BYF1 ACE2 DKFZP434A014 300335 59272 ACEH |
|---|---|
| Target | ACE2 |
| Cellular Localization | Nuclear |
Application
Immunofluorescence of ACE2 in Human Kidney cells with ACE2 antibody at 10 ug/mL.
Application
Western blot analysis of ACE2 in human kidney lysate with ACE2 antibody at 0.5 (lane A), 1 (lane B), and 2 (lane C) μg/mL, respectively.
Application
Immunohistochemical staining of human kidney tissue using ACE2 antibody at 2 μg/mL.| Positive Control | Human kidney or human testis cell lysate |
|---|---|
| Application Notes | For IHC-P: Use at a concentration of 2ug/ml. WB: Use at a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 97 kDa). Peptide available as GTX15352. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, containing 0.02% sodium azide |
