Application
IHC-P analysis of brain tissue using Acetyl CoA Carboxylase 1 (ACC1) antibody [EPR4971] at a dilution of 1:250.Application
WB analysis of (1) 293T, (2) HepG2 and (3) SH-SY5Y lysates (10 μg per lane) using Acetyl CoA Carboxylase 1 (ACC1) antibody [EPR4971] at a dilution of 1:1,000.Application
ICC/IF analysis of 293 cells using Acetyl CoA Carboxylase 1 (ACC1) antibody [EPR4971] at a dilution of 1:100.| Product Name | Acetyl-CoA Carboxylase 1 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit monoclonal antibody [EPR4971] to Acetyl-CoA Carboxylase 1Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen | The immunogen corresponding to a region within amino acids 1 and 40 of human Acetyl-CoA carboxylase 1 (Uniprot: Q13085). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | EPR4971 |
| Host Species | Rabbit |
| Tested Applications | FACSICC/IFIHC-PWB |
| FACS:1:200~1:500 ICC/IF:1:200~1:500 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Gene Synonyms | ACACA ACC ACACAD ACC1 ACAC ACCA |
|---|---|
| Target | Acetyl CoA Carboxylase 1 |
Application
IHC-P analysis of brain tissue using Acetyl CoA Carboxylase 1 (ACC1) antibody [EPR4971] at a dilution of 1:250.
Application
WB analysis of (1) 293T, (2) HepG2 and (3) SH-SY5Y lysates (10 μg per lane) using Acetyl CoA Carboxylase 1 (ACC1) antibody [EPR4971] at a dilution of 1:1,000.
Application
ICC/IF analysis of 293 cells using Acetyl CoA Carboxylase 1 (ACC1) antibody [EPR4971] at a dilution of 1:100.| Application Notes | FACS:1:200~1:500 ICC/IF:1:200~1:500 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for 12 months at -20°C |
| Storage Buffer | PBS containing 50% Glycerol, 0.05% BSA and 0.01% Sodium azide. |
