Application
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-Acetyl CoA Carboxylase 1 RM232 at a 1:300 dilution.Application
Western Blot of A431 cell lysates using Anti-Acetyl CoA Carboxylase 1 RM232 at a 1: 1,000 dilution, showed a band of Acetyl CoA Carboxylase 1 (~260 kDa) expressed in A431 cells.| Product Name | Acetyl-CoA Carboxylase 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Monoclonal antibody [RM232] to Acetyl-CoA Carboxylase 1Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen | A peptide corresponding to human Acetyl CoA Carboxylase 1. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | RM232 |
| Host Species | Rabbit |
| Tested Applications | IHCIHC-PWB |
| Immunohistochemistry (IHC): 1:300-1:500 dilution, Western Blot (WB): 1:1000-1:2000 dilution.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ACACAD ACCA acetyl-CoA carboxylase alpha ACACA ACC1 ACAC ACC |
|---|---|
| Target | Acetyl-CoA Carboxylase |
| Tissue Specificity | This antibody reacts to human Acetyl CoA Carboxylase 1. This antibody may also react to mouse or rat Acetyl CoA Carboxylase 1, as predicted by immunogen homology. |
Application
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-Acetyl CoA Carboxylase 1 RM232 at a 1:300 dilution.
Application
Western Blot of A431 cell lysates using Anti-Acetyl CoA Carboxylase 1 RM232 at a 1: 1,000 dilution, showed a band of Acetyl CoA Carboxylase 1 (~260 kDa) expressed in A431 cells.| Application Notes | Immunohistochemistry (IHC): 1:300-1:500 dilution, Western Blot (WB): 1:1000-1:2000 dilution.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20.0°C |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
