Application
Western blot analysis of CHRNA1 expression in HeLa (A); MCF7 (B); mouse kidney (C); rat liver (D) whole cell lysates.Application
Immunohistochemical analysis of CHRNA1 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Product Name | AChR alpha 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to AChR alpha 1The muscle acetylcholine receptor consiststs of 5 subunits of 4 different types: 2 alpha subunits and 1 each of the beta, gamma, and delta subunits. This gene encodes an alpha subunit that plays a role in acetlycholine binding/channel gating. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Nov 2012] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CHRNA1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIHC-PWB |
| IHC:1:50~1:200 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseDogRatBovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FCCMS CHRNA CHRNA1 ACHRD CMS2A SCCMS cholinergic receptor nicotinic alpha 1 subunit ACHRA |
|---|---|
| Target | AChR alpha 1 |
| Tissue Specificity | Recognizes endogenous levels of CHRNA1 protein. |
Application
Western blot analysis of CHRNA1 expression in HeLa (A); MCF7 (B); mouse kidney (C); rat liver (D) whole cell lysates.
Application
Immunohistochemical analysis of CHRNA1 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Application Notes | IHC:1:50~1:200 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 C. Upon delivery aliquot and store at -20 C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide. |
