Application
WB analysis of HepG2, Raji cells using AChR alpha 2 antibody at 0.5μg/ml. Lane A: HepG2 cell lysate Lane B: Raji cell lysate| Product Name | AChR alpha 2 antibody, N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to AChR alpha 2Nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels formed by a pentameric arrangement of alpha and beta subunits to create distinct muscle and neuronal receptors. Neuronal receptors are found throughout the peripheral and central nervous system where they are involved in fast synaptic transmission. This gene encodes an alpha subunit that is widely expressed in the brain. The proposed structure for nAChR subunits is a conserved N-terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region. Mutations in this gene cause autosomal dominant nocturnal frontal lobe epilepsy type 4. Single nucleotide polymorphisms (SNPs) in this gene have been associated with nicotine dependence. [provided by RefSeq, Nov 2009] |
| Immunogen | A synthetic peptide corresponding to a N-terminal region of Human AChR alpha 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Human: 100% |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | CHRNA2 |
|---|---|
| Target | AChR alpha 2 |
| Molecular Weight(MW) | 60 KDa (note) |
Application
WB analysis of HepG2, Raji cells using AChR alpha 2 antibody at 0.5μg/ml. Lane A: HepG2 cell lysate Lane B: Raji cell lysate| Positive Control | HepG2 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. For short-term storage, store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
