Application
Sample (30 ug of whole cell lysate) A: Molt-4 7.5% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of methanol-fixed A431, using Nicotinic Acetylcholine Receptor alpha 3 antibody at 1:500 dilution.| Product Name | AChR alpha 3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to AChR alpha 3This locus encodes a member of the nicotinic acetylcholine receptor family of proteins. Members of this family of proteins form pentameric complexes comprised of both alpha and beta subunits. This locus encodes an alpha-type subunit, as it contains characteristic adjacent cysteine residues. The encoded protein is a ligand-gated ion channel that likely plays a role in neurotransmission. Polymorphisms in this gene have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer. Alternatively spliced transcript variants have been described. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Nicotinic Acetylcholine Receptor alpha 3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Chicken, BovineMouse (94%), Rat (94%), Chicken (87%), Bovine (96%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | LNCR2 antibody MGC104879 antibody NACHRA3 antibody PAOD2 antibody CHRNA3 antibody "neuronal nicotinic acetylcholine receptor alpha3 subunit antibody" neuronal acetylcholine receptor subunit alpha-3 antibody "cholinergic receptor nicotinic alpha p |
|---|---|
| Target | AChR alpha 3 |
| Molecular Weight(MW) | 57 kDa (note) (2) |
| Cellular Localization | Cell junction , synapse , postsynaptic cell membrane , Cell membrane |
Application
Sample (30 ug of whole cell lysate) A: Molt-4 7.5% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of methanol-fixed A431, using Nicotinic Acetylcholine Receptor alpha 3 antibody at 1:500 dilution.| Positive Control | Molt-4 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
