Application
Immunoprecipitation of Aconitase 2 protein from HeLa whole cell extracts using 5 μg of Aconitase 2 antibody [C1C3] . Western blot analysis was performed using Aconitase 2 antibody [C1C3] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 7.5% SDS PAGE diluted at 1:10000Application
Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Aconitase 2 antibody [C1C3] diluted at 1:10000.Application
Aconitase 2 antibody [C1C3] detects Aconitase 2 protein at mitochondria on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. Aconitase 2 antibody [C1C3] dilution: 1:500.Application
Sample (20 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE diluted at 1:20000Application
Aconitase 2 antibody [C1C3] detects Aconitase 2 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Aconitase 2 protein stained by Aconitase 2 antibody [C1C3] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | Aconitase 2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Aconitase 2 (aconitase 2, mitochondrial)The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Aconitase 2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Pig, Chicken, Rhesus Monkey, BovineXenopus laevis (86%), Pig (96%), Chicken (92%), Rhesus Monkey (99%), Bovine (96%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ACONM antibody MGC20605 antibody MGC33908 antibody ACO2 antibody citrate hydro-lyase antibody "aconitate hydratase mitochondrial antibody" "aconitase 2 mitochondrial antibody" |
|---|---|
| Molecular Weight(MW) | 85 kDa (note) |
| Cellular Localization | Mitochondrion |
Application
Immunoprecipitation of Aconitase 2 protein from HeLa whole cell extracts using 5 μg of Aconitase 2 antibody [C1C3] . Western blot analysis was performed using Aconitase 2 antibody [C1C3] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 7.5% SDS PAGE diluted at 1:10000
Application
Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Aconitase 2 antibody [C1C3] diluted at 1:10000.
Application
Aconitase 2 antibody [C1C3] detects Aconitase 2 protein at mitochondria on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. Aconitase 2 antibody [C1C3] dilution: 1:500.
Application
Sample (20 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE diluted at 1:20000
Application
Aconitase 2 antibody [C1C3] detects Aconitase 2 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Aconitase 2 protein stained by Aconitase 2 antibody [C1C3] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
