Application
Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE diluted at 1:500Application
ACOT9 antibody [N1C1] detects ACOT9 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ACOT9 protein stained by ACOT9 antibody [N1C1] diluted at 1:500. Blue: Hoechst 33342 staining.Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using ACOT9 antibody at 1:250 dilution.| Product Name | ACOT9 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ACOT9 (acyl-CoA thioesterase 9)The protein encoded by this gene is a mitochondrial acyl-CoA thioesterase of unknown function. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ACOT9. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (81%), Rat (81%), Bovine (90%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ACATE2 antibody MT-ACT48 antibody ACOT9 antibody acyl-CoA thioester hydrolase 9 antibody "acyl-Coenzyme A thioesterase 2 mitochondrial antibody" mitochondrial Acyl-CoA Thioesterase antibody "acyl-coenzyme A thioesterase 9 mitochondrial antibody" a |
|---|---|
| Molecular Weight(MW) | 50 kDa (note) (2) |
| Cellular Localization | Mitochondrion |
Application
Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE diluted at 1:500
Application
ACOT9 antibody [N1C1] detects ACOT9 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ACOT9 protein stained by ACOT9 antibody [N1C1] diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using ACOT9 antibody at 1:250 dilution.| Positive Control | NT2D1 , U87-MG |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
