Application
Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE Lysosomal acid phosphatase2 antibody diluted at 1:1000Application
ACP2 antibody [N2C3] detects ACP2 protein at lysosome on Ca922 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Ca922 xenograft. ACP2 antibody [N2C3] dilution: 1:500.Application
Immunofluorescence analysis of methanol-fixed HeLa, using ACP2 antibody at 1:200 dilution.| Product Name | ACP2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ACP2 (acid phosphatase 2, lysosomal)This gene encodes the beta subunit of lysosomal acid phosphatase (LAP). LAP is chemically and genetically distinct from red cell acid phosphatase. The encoded protein belongs to a family of distinct isoenzymes which hydrolyze orthophosphoric monoesters to alcohol and phosphate. Mutations in this gene or in the related alpha subunit gene cause acid phosphatase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ACP2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, Chimpanzee, BovineMouse (87%), Rat (87%), Pig (94%), Chimpanzee (100%), Bovine (94%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ACP2 antibody LAP antibody lysosomal acid phosphatase antibody "acid phosphatase 2 lysosomal antibody" |
|---|---|
| Molecular Weight(MW) | 48 kDa (note) (4) |
| Cellular Localization | Lysosome membrane; Single-pass membrane protein; Lumenal side , Lysosome lumen |
Application
Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE Lysosomal acid phosphatase2 antibody diluted at 1:1000
Application
ACP2 antibody [N2C3] detects ACP2 protein at lysosome on Ca922 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Ca922 xenograft. ACP2 antibody [N2C3] dilution: 1:500.
Application
Immunofluorescence analysis of methanol-fixed HeLa, using ACP2 antibody at 1:200 dilution.| Positive Control | A431 , H1299 , HeLa , HepG2 , *H3347 , *CX-1 , *SW48 , *HCT116 , *Human Lymphoma Tissue |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
