Application
ADAMTS5 antibody [N3C2], Internal detects ADAMTS5 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded human hepatoma. ADAMTS5 antibody [N3C2], Internal dilution: 1:250. Scale bar = 100 μm.Application
Sample (30 ug of whole cell lysate) A: HeLa B: HepG2 7.5% SDS PAGE diluted at 1:3000Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using ADAMTS5 antibody at 1:500 dilution.Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAMTS5 antibody [N3C2], Internal diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | ADAMTS5 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ADAMTS5 (ADAM metallopeptidase with thrombospondin type 1 motif, 5)This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene contains two C-terminal TS motifs and functions as aggrecanase to cleave aggrecan, a major proteoglycan of cartilage. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ADAMTS5. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, BovineMouse (98%), Rat (98%), Zebrafish (84%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ADAMTS11 antibody ADMP-2 antibody FLJ36738 antibody ADAMTS5 antibody a disintegrin and metalloproteinase with thrombospondin motifs 11 antibody A disintegrin and metalloproteinase with thrombospondin motifs 5 antibody "a disintegrin-like and metallop |
|---|---|
| Molecular Weight(MW) | 102 kDa (note) (2) |
Application
ADAMTS5 antibody [N3C2], Internal detects ADAMTS5 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded human hepatoma. ADAMTS5 antibody [N3C2], Internal dilution: 1:250. Scale bar = 100 μm.
Application
Sample (30 ug of whole cell lysate) A: HeLa B: HepG2 7.5% SDS PAGE diluted at 1:3000
Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using ADAMTS5 antibody at 1:500 dilution.
Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAMTS5 antibody [N3C2], Internal diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
