Application
Sample (30 ug of whole cell lysate) A: NT2D1 7.5% SDS PAGE diluted at 1:1000Application
ADAR2 antibody detects ADAR2 protein at nucleus by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ADAR2 protein stained by ADAR2 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | ADAR2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ADAR2 (double-stranded RNA-specific editase 1)This gene encodes the enzyme responsible for pre-mRNA editing of the glutamate receptor subunit B by site-specific deamination of adenosines. Studies in rat found that this enzyme acted on its own pre-mRNA molecules to convert an AA dinucleotide to an AI dinucleotide which resulted in a new splice site. Alternative splicing of this gene results in several transcript variants, some of which have been characterized by the presence or absence of an ALU cassette insert and a short or long C-terminal region. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ADAR2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Chicken, ChimpanzeeMouse (96%), Rat (96%), Chicken (82%), Chimpanzee (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ADAR2 antibody DRABA2 antibody DRADA2 antibody RED1 antibody ADARB1 antibody "adenosine deaminase RNA-specific B1 (homolog of rat RED1) antibody" dsRNA adenosine deaminase antibody "adenosine deaminase RNA-specific B1 (RED1 homolog rat) antibody |
|---|---|
| Molecular Weight(MW) | 81 kDa (note) (5) |
Application
Sample (30 ug of whole cell lysate) A: NT2D1 7.5% SDS PAGE diluted at 1:1000
Application
ADAR2 antibody detects ADAR2 protein at nucleus by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ADAR2 protein stained by ADAR2 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | NT2D1 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
