Application
Immunohistochemistry-Paraffin: Adenosine A2a Receptor Antibody - showing positive staining in the cytoplasm and membrane of paraffin-treated Mouse testis tissue (right) compared with a negative control in the absence of primary antibody (left).| Product Name | Adenosine A2a Receptor antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Adenosine Receptor A2aAdenosine receptors (ARs) are members of the 7-transmembrane domain G-protein-coupled receptor superfamily. Structural, biochemical and pharmacological analyses of the AR genes and protein has led to the discovery of four distinct AR subtypes (A1, A2a, A2b, A3). Activation of ARs mediates several receptor subtype-specific physiological processes that include cardiac rate, smooth muscle tone, platelet aggregation, inflammation, cell growth and death, and neurotransmission. The A2aAR is a glycoprotein that can activate adenylyl cyclase via Gs interactions. Stimulation of A2aAR and the resultant accumulation of cAMP inhibits platelet aggregation and in certain vascular beds is associated with vasodilation and drop in blood pressure. A2aAR is reported to interact with D2 dopamine receptor sites in the brain where they co-express (striatum, olfactory tubercle and nucleus accumbens). Other tissues including heart, kidney, and lung also express A2aAR. |
| Immunogen | Synthetic peptide: ESHGDMGLPDVELLSHELK, corresponding to amino acids 373-391 of Dog Adenosine Receptor A2a. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PIPWB |
| Immunohistochemistry: use at a dilution of 1/100. Immunoprecipitation: assay dependent. Western Blot: use at a dilution of 1/2000. Optimal dilution / concentration should be determined by the end user. By Western blot, this antibody detects an ~45 kDa protein from canine striatum representing A2aAR. Immunohistochemical staining of A2aAR in human hippocampus yields a pattern consistent with plasma membrane staining.: |
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| Species Reactivity | HumanDog |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ADORA2 RDC8 hA2aR 102776 ADORA2A P29274 135 Adenosine receptor A2a |
|---|---|
| Target | Adenosine receptor A2a |
| Tissue Specificity | Detects adenosine receptor A2a. This antibody does not detect other AR subtypes. |
| Cellular Localization | Cell Membrane |
Application
Immunohistochemistry-Paraffin: Adenosine A2a Receptor Antibody - showing positive staining in the cytoplasm and membrane of paraffin-treated Mouse testis tissue (right) compared with a negative control in the absence of primary antibody (left).| Application Notes | Immunohistochemistry: use at a dilution of 1/100. Immunoprecipitation: assay dependent. Western Blot: use at a dilution of 1/2000. Optimal dilution / concentration should be determined by the end user. By Western blot, this antibody detects an ~45 kDa protein from canine striatum representing A2aAR. Immunohistochemical staining of A2aAR in human hippocampus yields a pattern consistent with plasma membrane staining.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS, 1 mg/ml BSA and 0.05% sodium azide |
