Application
Adenylate kinase 2 antibody [N1C3] detects Adenylate kinase 2 protein at mitochondria in rat pancreas by immunohistochemical analysis. Sample: Paraffin-embedded rat pancreas. Adenylate kinase 2 antibody [N1C3] diluted at 1:500.Application
Adenylate kinase 2 antibody [N1C3] detects Adenylate kinase 2 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: Adenylate kinase 2 protein stained by Adenylate kinase 2 antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining.Application
Adenylate kinase 2 antibody [N1C3] detects Adenylate kinase 2 protein at mitochondria in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. Adenylate kinase 2 antibody [N1C3] diluted at 1:500.Application
Sample (30 ug of whole cell lysate) A: HeLa 12% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Adenylate kinase 2 antibody at 1:500 dilution.Application
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000| Product Name | Adenylate kinase 2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Adenylate kinase 2 (adenylate kinase 2)Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Three isozymes of adenylate kinase, namely 1, 2, and 3, have been identified in vertebrates; this gene encodes isozyme 2. Expression of these isozymes is tissue-specific and developmentally regulated. Isozyme 2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Adenylate kinase 2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Rhesus Monkey, BovineRhesus Monkey (100%), Bovine (93%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ADK2 antibody AK 2 antibody AK2 antibody ATP-AMP transphosphorylase 2 antibody "adenylate kinase 2 mitochondrial antibody" "adenylate kinase isoenzyme 2 mitochondrial antibody" adenylate kinase 2 antibody |
|---|---|
| Molecular Weight(MW) | 26 kDa (note) (5) |
| Cellular Localization | Mitochondrion intermembrane space |
Application
Adenylate kinase 2 antibody [N1C3] detects Adenylate kinase 2 protein at mitochondria in rat pancreas by immunohistochemical analysis. Sample: Paraffin-embedded rat pancreas. Adenylate kinase 2 antibody [N1C3] diluted at 1:500.
Application
Adenylate kinase 2 antibody [N1C3] detects Adenylate kinase 2 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: Adenylate kinase 2 protein stained by Adenylate kinase 2 antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Adenylate kinase 2 antibody [N1C3] detects Adenylate kinase 2 protein at mitochondria in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. Adenylate kinase 2 antibody [N1C3] diluted at 1:500.
Application
Sample (30 ug of whole cell lysate) A: HeLa 12% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Adenylate kinase 2 antibody at 1:500 dilution.
Application
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
