Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using ADHFE1 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: U87-MG 12% SDS PAGE diluted at 1:1000Application
ADHFE1 antibody [N1C1] detects ADHFE1 protein at mitochondria by immunofluorescent analysis. Sample: Hep G2 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: ADHFE1 protein stained by ADHFE1 antibody [N1C1] diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | ADHFE1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ADHFE1 (alcohol dehydrogenase, iron containing, 1)The ADHFE1 gene encodes hydroxyacid-oxoacid transhydrogenase (EC 1.1.99.24), which is responsible for the oxidation of 4-hydroxybutyrate in mammalian tissues (Kardon et al., 2006 [PubMed 16616524]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ADHFE1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (89%), Rat (89%), Bovine (88%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ADH8 antibody FLJ32430 antibody HOT antibody MGC48605 antibody ADHFE1 antibody alcohol dehydrogenase 8 antibody Fe-containing alcohol dehydrogenase 1 antibody alcohol dehydrogenase iron-containing protein 1 antibody "hydroxyacid-oxoacid transhydrog |
|---|---|
| Molecular Weight(MW) | 50 kDa (note) (2) |
| Cellular Localization | Mitochondrion |
Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using ADHFE1 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: U87-MG 12% SDS PAGE diluted at 1:1000
Application
ADHFE1 antibody [N1C1] detects ADHFE1 protein at mitochondria by immunofluorescent analysis. Sample: Hep G2 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: ADHFE1 protein stained by ADHFE1 antibody [N1C1] diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | NT2D1 , IMR32 , U87-MG , MCF-7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
