Application
AF9 antibody detects AF9 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AF9 protein stained by AF9 antibody diluted at 1:1000. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar = 10 μm.Application
Sample (30 µg of whole cell lysate) A: THP-1 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded D54 xenograft, using AF9 antibody at 1:750 dilution.| Product Name | AF9 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to AF9 (myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 3) |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human AF9. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWBChIP |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (98%), Rat (98%), Bovine (97%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AF9 antibody, FLJ2035 antibody, YEATS3 antibody, MLLT3 antibody, "myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog), translocated to, 3 antibody", ALL1-fused gene from chromosome 9 protein antibody, YEATS domain-containing protein 3 antibody, |
|---|---|
| Molecular Weight(MW) | 63 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
AF9 antibody detects AF9 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AF9 protein stained by AF9 antibody diluted at 1:1000. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar = 10 μm.
Application
Sample (30 µg of whole cell lysate) A: THP-1 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded D54 xenograft, using AF9 antibody at 1:750 dilution.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
