Application
Sample (30 ug of whole cell lysate) A: H1299 B: Hep G2 7.5% SDS PAGE diluted at 1:1000Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AIF antibody [N1C1] diluted at 1:1000.Application
Immunohistochemical analysis of paraffin-embedded SW480 Xenograft, using AIF antibody at 1:100 dilution.Application
Immunofluorescence analysis of methanol-fixed HeLa, using AIF antibody at 1:200 dilution.Application
Sample (50 ug of whole cell lysate) A: mouse liver 7.5% SDS PAGE diluted at 1:1000Application
Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AIF antibody [N1C1] diluted at 1:1000.| Product Name | AIF antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to AIF (apoptosis-inducing factor, mitochondrion-associated, 1)This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells that is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it effects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Several alternative transcripts encoding different isoforms have been identified for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human AIF. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Pig, Chicken, Rhesus Monkey, BovineZebrafish (80%), Pig (97%), Chicken (84%), Rhesus Monkey (99%), Bovine (95%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AIF antibody, COXPD6 antibody, MGC111425 antibody, PDCD8 antibody, AIFM1 antibody, striatal apoptosis-inducing factor antibody, "apoptosis-inducing factor 1, mitochondrial antibody", programmed cell death 8 (apoptosis-inducing factor) antibody, "apoptosis- |
|---|---|
| Molecular Weight(MW) | 67 kDa (note) (4) |
| Cellular Localization | Mitochondrion intermembrane space , Nucleus |
Application
Sample (30 ug of whole cell lysate) A: H1299 B: Hep G2 7.5% SDS PAGE diluted at 1:1000
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AIF antibody [N1C1] diluted at 1:1000.
Application
Immunohistochemical analysis of paraffin-embedded SW480 Xenograft, using AIF antibody at 1:100 dilution.
Application
Immunofluorescence analysis of methanol-fixed HeLa, using AIF antibody at 1:200 dilution.
Application
Sample (50 ug of whole cell lysate) A: mouse liver 7.5% SDS PAGE diluted at 1:1000
Application
Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AIF antibody [N1C1] diluted at 1:1000.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
