Application
AIMP1 antibody detects AIMP1 protein at cytosol on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. AIMP1 antibody dilution: 1:500.Application
AIMP1 antibody detects AIMP1 protein at cytoplasm in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. AIMP1 antibody diluted at 1:500.Application
Sample (30 ug of whole cell lysate) A: Hela B: JurKat 10% SDS PAGE diluted at 1:1000Application
AIMP1 antibody detects SCYE1 protein at Cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SCYE1 protein stained by AIMP1 antibody diluted at 1:500. Blue: Hoechst 33343 staining.Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 10% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using AIMP1 antibody at 1:100 dilution.| Product Name | AIMP1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to AIMP1 (aminoacyl tRNA synthetase complex-interacting multifunctional protein 1)The protein encoded by this gene is a cytokine that is specifically induced by apoptosis, and it is involved in the control of angiogenesis, inflammation, and wound healing. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor protein is identical to the p43 subunit, which is associated with the multi-tRNA synthetase complex, and it modulates aminoacylation activity of tRNA synthetase in normal cells. This protein is also involved in the stimulation of inflammatory responses after proteolytic cleavage in tumor cells. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene has been identified on chromosome 20. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human AIMP1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Pig, BovineRat (97%), Zebrafish (83%), Xenopus laevis (85%), Pig (94%), Bovine (94%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | EMAP2 antibody, EMAPII antibody, SCYE1 antibody, p43 antibody, AIMP1 antibody, "small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating) antibody", endothelial-monocyte activating polypeptide II antibody, aminoacyl tRNA synthase com |
|---|---|
| Molecular Weight(MW) | 34 kDa (note) (4) |
| Cellular Localization | Nucleus , Cytoplasm , cytosol , Cytoplasmic vesicle , secretory vesicle , Secreted , Endoplasmic reticulum , Golgi apparatus |
Application
AIMP1 antibody detects AIMP1 protein at cytosol on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. AIMP1 antibody dilution: 1:500.
Application
AIMP1 antibody detects AIMP1 protein at cytoplasm in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. AIMP1 antibody diluted at 1:500.
Application
Sample (30 ug of whole cell lysate) A: Hela B: JurKat 10% SDS PAGE diluted at 1:1000
Application
AIMP1 antibody detects SCYE1 protein at Cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SCYE1 protein stained by AIMP1 antibody diluted at 1:500. Blue: Hoechst 33343 staining.
Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 10% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using AIMP1 antibody at 1:100 dilution.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
