Application
Sample (30 ug of whole cell lysate) A: HeLa 7.5% SDS PAGE diluted at 1:10000Application
Immunofluorescence analysis of methanol-fixed HeLa, using AKAP1 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using AKAP1 antibody at 1:250 dilution.| Product Name | AKAP1 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to AKAP1 (A kinase (PRKA) anchor protein 1)The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein binds to type I and type II regulatory subunits of PKA and anchors them to the mitochondrion. This protein is speculated to be involved in the cAMP-dependent signal transduction pathway and in directing RNA to a specific cellular compartment. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human AKAP1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Rhesus Monkey, ChimpanzeeRhesus Monkey (85%), Chimpanzee (97%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AKAP antibody AKAP121 antibody AKAP149 antibody AKAP84 antibody D-AKAP1 antibody MGC1807 antibody PRKA1 antibody SAKAP84 antibody AKAP1 antibody AKAP 149 antibody "protein phosphatase 1 regulatory subunit 43 antibody" dual-specificity A-kinase |
|---|---|
| Molecular Weight(MW) | 97 kDa (note) (4) |
| Cellular Localization | Mitochondrion outer membrane |
Application
Sample (30 ug of whole cell lysate) A: HeLa 7.5% SDS PAGE diluted at 1:10000
Application
Immunofluorescence analysis of methanol-fixed HeLa, using AKAP1 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using AKAP1 antibody at 1:250 dilution.| Positive Control | A549 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
