Application
Sample (30μg whole cell lysate) A:HeLa S3 5% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using AKAP12 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded human serous ovarian cancer, using AKAP12 antibody at 1:100 dilution.| Product Name | AKAP12 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to AKAP12 (A kinase (PRKA) anchor protein (gravin) 12)The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human AKAP12. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Rhesus MonkeyRhesus Monkey (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AKAP250 antibody DKFZp686M0430 antibody DKFZp686O0331 antibody FLJ20945 antibody FLJ97621 antibody AKAP12 antibody Src-Suppressed C Kinase Substrate antibody "A-kinase anchor protein 250kDa antibody" myasthenia gravis autoantigen gravin antibody |
|---|---|
| Target | AKAP12 |
| Molecular Weight(MW) | 191 kDa (note) (2) |
| Cellular Localization | Cytoplasm , cell cortex , cytoskeleton |
Application
Sample (30μg whole cell lysate) A:HeLa S3 5% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using AKAP12 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded human serous ovarian cancer, using AKAP12 antibody at 1:100 dilution.| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
