Application
Western blot analysis of phosphorylated Akt in NIH3T3 cells without (left lane) or with (right lane) induction by PDGF.| Product Name | AKT (phospho Ser473) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Akt (phospho Ser473)Akt, also referred to as PKB and RAC, is the cellular homologue of the viral oncogene v-Akt. This protein kinase is activated by insulin and various growth factors and functions in a pathway involving PI3 kinase as activation is sensitive to wortmanin. Recent evidence suggests that Akt functions to promote cell survival by actively inhibiting apoptosis, although the pathways involved have not been identified. Akt is activated by phospholipid binding and phosphorylation within the activation loop at Thr308 and within the C-terminus at Ser473. |
| Modification | p-Ser473 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| Western blotting (0.5-4 µg/ml) and immunoprecipitation (10-20 µg/ml). However, the optimal conditions should be determined individually. The phospho-Akt (Ser473) antibody detects Akt1 when phosphorylated at Ser473 and Akt2 and Akt3 when phosphorylated at equivalent sites.: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Protein kinase B DRAC-PK RAC serine/threonine-protein kinase phospho Akt (pS473) phospho-A Dakt1 phosphoAkt (pS473) phospho Akt (pSer473) AKT1 DAkt RAC serine/threonineprotein kinase phospho-Akt (pSer473) phosphoAkt (pSer473) DRACPK phosphoA |
|---|---|
| Target | Akt (phospho Ser473) |
| Tissue Specificity | This antibody is specific for Akt (phospho Ser473). |
| Cellular Localization | Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. |
Application
Western blot analysis of phosphorylated Akt in NIH3T3 cells without (left lane) or with (right lane) induction by PDGF.| Application Notes | Western blotting (0.5-4 µg/ml) and immunoprecipitation (10-20 µg/ml). However, the optimal conditions should be determined individually. The phospho-Akt (Ser473) antibody detects Akt1 when phosphorylated at Ser473 and Akt2 and Akt3 when phosphorylated at equivalent sites.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The antibody solution should be gently mixed before use. Store at +4C. Product should remain stable for at least 12 months. |
| Storage Buffer | Phosphate buffered saline (PBS), containing 50% glycerol, 1% BSA, 0.02% thimerosal |
