Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using Akt (phospho-Thr308) antibody.Application
Western blot analysis using Akt (phospho-Thr308) antibody : Lane1: The extract from 293 cells untreated; Lane 2: The extract from 293 cells treated with insulin.| Product Name | AKT (phospho Thr308) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Akt (phospho T308)The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein plays a central role in exerting IL4 mediated biological responses. It is found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins. Alternative splicing results in multiple transcript variants. |
| Conjugation | Agarose |
| Modification | p-Thr308 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PWB |
| Suggested starting dilutions are as follows. IHC-P: 1:50-1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | D12S1644 IL-4-STAT STAT6B STAT6C |
|---|---|
| Target | Akt (phospho T308) |
| Cellular Localization | Translocated into the nucleus in response to phosphorylation. |
Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using Akt (phospho-Thr308) antibody.
Application
Western blot analysis using Akt (phospho-Thr308) antibody : Lane1: The extract from 293 cells untreated; Lane 2: The extract from 293 cells treated with insulin.| Application Notes | Suggested starting dilutions are as follows. IHC-P: 1:50-1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS (without Mg2+and Ca2+), pH 7.4, 150mM NaCl,0.02% sodium azide and 50% glycerol. |
