Application
Western blot analysis of ALAD Antibody (C-term) in Jurkat cell line lysates (35ug/lane). ALAD (arrow) was detected using the purified Pab.Application
ALAD Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with ALAD Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | ALAD antibody, C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to ALAD (C-term)The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway; zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria. [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 251~280 amino acids from the C-terminal region of human ALAD. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Gene Synonyms | ALAD ALADH Delta-aminolevulinic acid dehydratase Porphobilinogen synthase Deltaaminolevulinic acid dehydratase |
|---|---|
| Target | ALAD |
| Molecular Weight(MW) | 36.295 kDa (note) |
Application
Western blot analysis of ALAD Antibody (C-term) in Jurkat cell line lysates (35ug/lane). ALAD (arrow) was detected using the purified Pab.
Application
ALAD Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with ALAD Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
