Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HepG2 7.5% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using alanyl-tRNA synthetase antibody at 1:500 dilution.| Product Name | alanyl-tRNA synthetase antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to alanyl-tRNA synthetase (alanyl-tRNA synthetase, cytoplasmic)The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human alanyl-tRNA synthetase. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, Chicken, BovineMouse (96%), Rat (96%), Zebrafish (90%), Xenopus laevis (90%), Chicken (89%), Bovine (97%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CMT2N antibody AARS antibody "alanine tRNA ligase 1 cytoplasmic antibody" alanine--tRNA ligase antibody renal carcinoma antigen NY-REN-42 antibody alaRS antibody "alanyl-tRNA synthetase cytoplasmic antibody" alanyl-tRNA synthetase antibody |
|---|---|
| Molecular Weight(MW) | 107 kDa (note) |
| Cellular Localization | Cytoplasm |
Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HepG2 7.5% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using alanyl-tRNA synthetase antibody at 1:500 dilution.| Positive Control | 293T , A431 , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
