Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using ALDH1A1 antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: A549 10% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of methanol-fixed A549, using ALDH1A1 antibody at 1:500 dilution.Application
ALDH1A1 antibody detects ALDH1A1 protein by western blot analysis. A. 50 µg mouse liver lysate/extract 10% SDS-PAGE ALDH1A1 antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
ALDH1A1 antibody detects ALDH1A1 protein by western blot analysis. A. 50 µg rat liver lysate/extract 10% SDS-PAGE ALDH1A1 antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | ALDH1A1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ALDH1A1 (aldehyde dehydrogenase 1 family, member A1)This protein belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a cytosolic isoform, which has a high affinity for aldehydes. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ALDH1A1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Dog, Rabbit, Chicken, Sheep, Chimpanzee, BovineXenopus laevis (81%), Dog (90%), Rabbit (90%), Chicken (86%), Sheep (93%), Chimpanzee (100%), Bovine (93%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ALDC antibody ALDH-E1 antibody ALDH1 antibody ALDH11 antibody MGC2318 antibody PUMB1 antibody RALDH1 antibody ALDH1A1 antibody ALHDII antibody ALDH class 1 antibody retinaldehyde dehydrogenase 1 antibody acetaldehyde dehydrogenase 1 antibody al |
|---|---|
| Molecular Weight(MW) | 55 kDa (note) (2) |
| Cellular Localization | Cytoplasm |
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using ALDH1A1 antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: A549 10% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of methanol-fixed A549, using ALDH1A1 antibody at 1:500 dilution.
Application
ALDH1A1 antibody detects ALDH1A1 protein by western blot analysis. A. 50 µg mouse liver lysate/extract 10% SDS-PAGE ALDH1A1 antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
ALDH1A1 antibody detects ALDH1A1 protein by western blot analysis. A. 50 µg rat liver lysate/extract 10% SDS-PAGE ALDH1A1 antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | A549 , mouse liver , rat liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
