Application
Sample (30 µg of whole cell lysate) A: IMR32 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (50 µg of whole cell lysate) A: mouse liver 7.5% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
ALDH1A2 antibody detects ALDH1A2 protein by western blot analysis. Rat tissue extracts (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A2 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
ALDH1A2 antibody detects ALDH1A2 protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A2 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
ALDH1A2 antibody [C1C3] detects ALDH1A2 protein at cytoplasm in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. ALDH1A2 antibody [C1C3] diluted at 1:500.| Product Name | ALDH1A2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ALDH1A2 (aldehyde dehydrogenase 1 family, member A2)This protein belongs to the aldehyde dehydrogenase family of proteins. The product of this gene is an enzyme that catalyzes the synthesis of retinoic acid (RA) from retinaldehyde. Retinoic acid, the active derivative of vitamin A (retinol), is a hormonal signaling molecule that functions in developing and adult tissues. The studies of a similar mouse gene suggest that this enzyme and the cytochrome CYP26A1, concurrently establish local embryonic retinoic acid levels which facilitate posterior organ development and prevent spina bifida. Three transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ALDH1A2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Dog, ChickenXenopus laevis (91%), Dog (99%), Chicken (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC26444 antibody RALDH(II) antibody RALDH2 antibody RALDH2-T antibody ALDH1A2 antibody retinal dehydrogenase 2 antibody retinaldehyde-specific dehydrogenase type 2 antibody RALDH 2 antibody "aldehyde dehydrogenase 1 family member A2 antibody" |
|---|---|
| Molecular Weight(MW) | 57 kDa (note) (5) |
| Cellular Localization | Cytoplasm |
Application
Sample (30 µg of whole cell lysate) A: IMR32 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (50 µg of whole cell lysate) A: mouse liver 7.5% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
ALDH1A2 antibody detects ALDH1A2 protein by western blot analysis. Rat tissue extracts (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A2 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
ALDH1A2 antibody detects ALDH1A2 protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A2 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
ALDH1A2 antibody [C1C3] detects ALDH1A2 protein at cytoplasm in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. ALDH1A2 antibody [C1C3] diluted at 1:500.| Positive Control | IMR32 , mouse liver , mouse testis , rat testis |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
