Application
Sample (20 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE diluted at 1:10000Application
Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE diluted at 1:1000Application
ALDH2 antibody [N1C1] detects ALDH2 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: ALDH2 protein stained by ALDH2 antibody [N1C1] diluted at 1:500. Blue: Hoechst 33343 staining.| Product Name | ALDH2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ALDH2 (aldehyde dehydrogenase 2 family (mitochondrial))This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ALDH2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Pig, BovineRat (96%), Xenopus laevis (83%), Pig (92%), Bovine (93%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ALDH-E2 antibody ALDHI antibody ALDM antibody MGC1806 antibody ALDH2 antibody nucleus-encoded mitochondrial aldehyde dehydrogenase 2 antibody liver mitochondrial ALDH antibody "aldehyde dehydrogenase mitochondrial antibody" acetaldehyde dehydrogen |
|---|---|
| Molecular Weight(MW) | 56 kDa (note) (3) |
| Cellular Localization | Mitochondrion matrix |
Application
Sample (20 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE diluted at 1:10000
Application
Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE diluted at 1:1000
Application
ALDH2 antibody [N1C1] detects ALDH2 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: ALDH2 protein stained by ALDH2 antibody [N1C1] diluted at 1:500. Blue: Hoechst 33343 staining.| Positive Control | A431 , HeLa , HepG2 , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
