Application
Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE diluted at 1:1000Application
ALDH5A1 antibody [N1C1] detects ALDH5A1 protein at mitochondria in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ALDH5A1 antibody [N1C1] diluted at 1:500.Application
Sample (20 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE diluted at 1:5000Application
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ALDH5A1 antibody at 1:500 dilution.Application
Immunofluorescence analysis of methanol-fixed A431, using ALDH5A1 antibody at 1:500 dilution.| Product Name | ALDH5A1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ALDH5A1 (aldehyde dehydrogenase 5 family, member A1)This protein belongs to the aldehyde dehydrogenase family of proteins. This gene encodes a mitochondrial NAD(+)-dependent succinic semialdehyde dehydrogenase. A deficiency of this enzyme, known as 4-hydroxybutyricaciduria, is a rare inborn error in the metabolism of the neurotransmitter 4-aminobutyric acid (GABA). In response to the defect, physiologic fluids from patients accumulate GHB, a compound with numerous neuromodulatory properties. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ALDH5A1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, Chimpanzee, BovineRat (84%), Pig (85%), Chimpanzee (92%), Bovine (86%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | SSADH antibody SSDH antibody ALDH5A1 antibody "succinate-semialdehyde dehydrogenase mitochondrial antibody" aldehyde dehydrogenase family 5 member A1 antibody mitochondrial succinate semialdehyde dehydrogenase antibody NAD(+)-dependent succinic semi |
|---|---|
| Molecular Weight(MW) | 57 kDa (note) (2) |
| Cellular Localization | Mitochondrion |
Application
Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE diluted at 1:1000
Application
ALDH5A1 antibody [N1C1] detects ALDH5A1 protein at mitochondria in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ALDH5A1 antibody [N1C1] diluted at 1:500.
Application
Sample (20 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE diluted at 1:5000
Application
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ALDH5A1 antibody at 1:500 dilution.
Application
Immunofluorescence analysis of methanol-fixed A431, using ALDH5A1 antibody at 1:500 dilution.| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain , *A549 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
