Application
WB analysis of HepG2 lysates using ALK (phospho Tyr1604) antibody in the absence (left) or presence (right) of blocking peptide.Application
ICC/IF analysis of HeLa cells using ALK (phospho Tyr1604) antibody in the absence (left) or presence (right) of blocking peptide.Application
WB analysis Cos-7 lysates using ALK (phospho Tyr1604) antibody in the presence (left) or absence (right) of blocking peptide.Application
IHC-P analysis of human brain tissue using ALK (phospho Tyr1604) antibody in the absence (left) or presence (right) of blocking peptide.| Product Name | ALK (phospho Tyr1604) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ALK (phospho Tyr1604)This gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumourigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosome 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome 5), ALK/KIF5B (chromosome 10), ALK/CLTC (chromosome 17), ALK/TPM4 (chromosome 19), and ALK/MSN (chromosome X).[provided by RefSeq, Jan 2011] |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 1604 (G-H-Y(p)-E-D) derived from Human ALK. |
| Modification | p-Tyr1604 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB: 1:500-1:3000. IHC-P: 1:50-1:100. ICC/IF: 1:100-1:500.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ALK NBLST3 anaplastic lymphoma receptor tyrosine kinase CD246 |
|---|---|
| Target | ALK (phospho Tyr1604) |
| Molecular Weight(MW) | 176 KDa (note) |
| Tissue Specificity | The antibody detects endogenous levels of ALK only when phosphorylated at tyrosine 1604. |
Application
WB analysis of HepG2 lysates using ALK (phospho Tyr1604) antibody in the absence (left) or presence (right) of blocking peptide.
Application
ICC/IF analysis of HeLa cells using ALK (phospho Tyr1604) antibody in the absence (left) or presence (right) of blocking peptide.
Application
WB analysis Cos-7 lysates using ALK (phospho Tyr1604) antibody in the presence (left) or absence (right) of blocking peptide.
Application
IHC-P analysis of human brain tissue using ALK (phospho Tyr1604) antibody in the absence (left) or presence (right) of blocking peptide.| Application Notes | WB: 1:500-1:3000. IHC-P: 1:50-1:100. ICC/IF: 1:100-1:500.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20˚C |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
