Application
Sample (30 µg of whole cell lysate) A: HepG2 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | alpha 1 Antitrypsin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to alpha 1 Antitrypsin (serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1)The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human alpha 1 Antitrypsin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Rhesus MonkeyRhesus Monkey (92%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | A1A antibody A1AT antibody AAT antibody MGC23330 antibody MGC9222 antibody PI antibody PI1 antibody PRO2275 antibody alpha1AT antibody SERPINA1 antibody serpin A1 antibody alpha-1 protease inhibitor antibody "serine (or cysteine) proteinase inh |
|---|---|
| Target | alpha 1 Antitrypsin |
| Molecular Weight(MW) | 47 kDa (note) (11) |
| Cellular Localization | Secreted , Short peptide from AAT: Secreted , extracellular space , extracellular matrix |
Application
Sample (30 µg of whole cell lysate) A: HepG2 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | A549 , HeLa , HepG2 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
