Application
Sample (30 ug of whole cell lysate) A: 293T 7.5% SDS PAGE diluted at 1:5000Application
Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Catenin antibody diluted at 1:500.Application
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using alpha Catenin antibody at 1:250 dilution.| Product Name | alpha 1 Catenin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to alpha 1 Catenin (catenin (cadherin-associated protein), alpha 1, 102kDa)Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherence junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human alpha 1 Catenin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Rabbit, Rhesus Monkey, BovineZebrafish (93%), Xenopus laevis (96%), Rabbit (100%), Rhesus Monkey (99%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CAP102 antibody FLJ36832 antibody FLJ52416 antibody CTNNA1 antibody "cadherin-associated protein 102kDa antibody" alpha-catenin antibody alphaE-catenin antibody catenin alpha-1 antibody renal carcinoma antigen NY-REN-13 antibody alpha-E-catenin an |
|---|---|
| Molecular Weight(MW) | 100 kDa (note) (32) |
| Cellular Localization | Cytoplasm , cytoskeleton , Cell junction , adherens junction , Cell membrane |
Application
Sample (30 ug of whole cell lysate) A: 293T 7.5% SDS PAGE diluted at 1:5000
Application
Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Catenin antibody diluted at 1:500.
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using alpha Catenin antibody at 1:250 dilution.| Positive Control | 293T , A431 , mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
