Application
alpha 1 Catenin antibody [N3C2], Internal detects alpha 1 Catenin protein at membrane on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. alpha 1 Catenin antibody [N3C2], Internal dilution: 1:500.Application
alpha 1 Catenin antibody [N3C2], Internal detects CTNNA1 protein at junction by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CTNNA1 protein stained by alpha 1 Catenin antibody [N3C2], Internal diluted at 1:500. Blue: Hoechst 33342 staining.Application
alpha 1 Catenin antibody [N3C2], Internal detects alpha 1 Catenin protein at membrane on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. alpha 1 Catenin antibody [N3C2], Internal dilution: 1:500.Application
Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE diluted at 1:1000Application
alpha 1 catenin antibody [N3c2], Internal detects alpha 1 Catenin protein at cytoplasm and cell junction by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha 1 Catenin protein stained by alpha 1 catenin antibody [N3c2], Internal diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:500. Blue: Hoechst 33342 staining.Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7.5% SDS PAGE diluted at 1:10000Application
Sample (30 ug of whole cell lysate) A: PC-12 7.5% SDS PAGE diluted at 1:10000| Product Name | alpha 1 Catenin antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to alpha 1 Catenin (catenin alpha-1)Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherence junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human alpha 1 Catenin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-FIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRatZebra Fish |
| Predicted Reactivity | Xenopus laevis, Rabbit, Rhesus Monkey, BovineXenopus laevis (93%), Rabbit (100%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CAP102 antibody FLJ36832 antibody FLJ52416 antibody CTNNA1 antibody "cadherin-associated protein 102kDa antibody" alpha-catenin antibody alphaE-catenin antibody catenin alpha-1 antibody renal carcinoma antigen NY-REN-13 antibody alpha-E-catenin an |
|---|---|
| Molecular Weight(MW) | 100 kDa (note) (32) |
| Cellular Localization | Cytoplasm , cytoskeleton , Cell junction , adherens junction , Cell membrane |
Application
alpha 1 Catenin antibody [N3C2], Internal detects alpha 1 Catenin protein at membrane on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. alpha 1 Catenin antibody [N3C2], Internal dilution: 1:500.
Application
alpha 1 Catenin antibody [N3C2], Internal detects CTNNA1 protein at junction by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CTNNA1 protein stained by alpha 1 Catenin antibody [N3C2], Internal diluted at 1:500. Blue: Hoechst 33342 staining.
Application
alpha 1 Catenin antibody [N3C2], Internal detects alpha 1 Catenin protein at membrane on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. alpha 1 Catenin antibody [N3C2], Internal dilution: 1:500.
Application
Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE diluted at 1:1000
Application
alpha 1 catenin antibody [N3c2], Internal detects alpha 1 Catenin protein at cytoplasm and cell junction by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha 1 Catenin protein stained by alpha 1 catenin antibody [N3c2], Internal diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7.5% SDS PAGE diluted at 1:10000
Application
Sample (30 ug of whole cell lysate) A: PC-12 7.5% SDS PAGE diluted at 1:10000| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , NIH-3T3 , JC , PC-12 |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
