Application
Human tissue extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha 2 Antiplasmin antibody [N3C3] diluted at 1:2000.Application
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using alpha 2 Antiplasmin antibody at 1:500 dilution.Application
Immunofluorescence analysis of methanol-fixed A431, using SERPINF2 antibody at 1:200 dilution.Application
Sample (50 ug of whole cell lysate) A: mouse liver 10% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE diluted at 1:1000| Product Name | alpha 2 Antiplasmin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to alpha 2 Antiplasmin (serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2)This gene encodes a member of the serpin family of serine protease inhibitors. The protein is a major inhibitor of plasmin, which degrades fibrin and various other proteins. Consequently, the proper function of this gene has a major role in regulating the blood clotting pathway. Mutations in this gene result in alpha-2-plasmin inhibitor deficiency, which is characterized by severe hemorrhagic diathesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human alpha 2 Antiplasmin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rabbit, BovineRabbit (86%), Bovine (83%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | A2AP antibody AAP antibody ALPHA-2-PI antibody API antibody PLI antibody SERPINF2 antibody serpin F2 antibody alpha-2-plasmin inhibitor antibody alpha-2-AP antibody alpha-2-antiplasmin antibody "serine (or cysteine) proteinase inhibitor clade F |
|---|---|
| Molecular Weight(MW) | 55 kDa (note) (3) |
| Cellular Localization | Secreted |
Application
Human tissue extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha 2 Antiplasmin antibody [N3C3] diluted at 1:2000.
Application
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using alpha 2 Antiplasmin antibody at 1:500 dilution.
Application
Immunofluorescence analysis of methanol-fixed A431, using SERPINF2 antibody at 1:200 dilution.
Application
Sample (50 ug of whole cell lysate) A: mouse liver 10% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse liver , human plasma |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
