Application
Alpha 2 adrenergic receptor labeling in rat cryostat section of rat brain. Labeling is confined to synaptic boutons (red). Tissue nuclei were counterstasined with DAPI (blue).| Product Name | alpha 2a Adrenergic receptor antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Alpha 2a Adrenergic ReceptorAlpha 2A, 2B, and 2C adrenergic receptors are seven transmembrane receptors that couple to inhibitory G-proteins. Alpha 2C has a wide distribution pattern in the CNS. On neurons Alpha 2 adrenergic receptors are located on both pre- and postsynaptic domains where they mediate an inhibitory role in the central and peripheral nervous system. Alpha 2C has a wide distribution pattern in the CNS. At the spinal cord level the vast majority of alpha 2C is not of primary afferent origin; however, a small fraction of neurons in DRG do express mRNA for alpha 2C and harbor the protein. Colocalization has been observed between alpha 2C and enkephalin in the spinal cord dorsal horn, suggesting that alpha 2C may be expressed by a subset of spinal interneurons. The distribution pattern of alpha 2C suggests functions at several levels in the brain. Antibody showed positive staining in rat spinal cord dorsal horn, ventral horn motor neurons and retina. When examined in other brain regions known to express ?2A staining was not observed. |
| Immunogen | Peptide - (C)KASRWRGRGNREKR (Cysteine added for conjugation) |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-F |
| For IHC-Fr: Use at a dilution of 1:100-1:500. Antiserum was used on perfusion fixed tissue. Perfusion: 1) calcium-free TyrodeÕs solution, 2) paraformaldehyde-picric acid fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Gene Synonyms | ADRA2A P08913 ADRA2 ADRAR 150 alpha2a Adrenergic receptor ZNF32 ALPHA2AAR ADRA2R alpha 2a Adrenergic receptor 104210 |
|---|---|
| Target | alpha 2a Adrenergic receptor |
Application
Alpha 2 adrenergic receptor labeling in rat cryostat section of rat brain. Labeling is confined to synaptic boutons (red). Tissue nuclei were counterstasined with DAPI (blue).| Application Notes | For IHC-Fr: Use at a dilution of 1:100-1:500. Antiserum was used on perfusion fixed tissue. Perfusion: 1) calcium-free TyrodeÕs solution, 2) paraformaldehyde-picric acid fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS. Optimal dilutions/concentrations should be determined by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon Receipt - Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
